5'-terminal cap structure in eucaryotic messenger ribonucleic acids.
نویسندگان
چکیده
منابع مشابه
Isolation of phi X174 specific messenger ribonucleic acids in vivo and identification of their 5' terminal nucleotides.
Deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) hybridization in formamide at low temperature was applied to hybridization of PhiX174 replicative form DNA and in vivo PhiX174 specific messenger RNA (mRNA) with some modification. We found that PhiX174 mRNA up to molecular weight 1.2 x 10(6) could be hybridized to and eluted from DNA without apparent breakage of phosphodiester bonds and th...
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A plasmid cDNA library was constructed using poly(A+) RNA isolated from the livers of rats treated with 3,5,3'-triiodothyronine (T3) and fed a high carbohydrate diet. This library was screened by differential colony hybridization with [32P]cDNA probes made from hypothyroid and hyperthyroid rat liver poly(A+) RNA to obtain clones representing T3-inducible mRNAs. Using plasmid cDNAs to 4 differen...
متن کاملCocrystal Structure of the Messenger RNA 5′ Cap-Binding Protein (eIF4E) Bound to 7-methyl-GDP
The X-ray structure of the eukaryotic translation initiation factor 4E (eIF4E), bound to 7-methyl-GDP, has been determined at 2.2 A resolution. eIF4E recognizes 5' 7-methyl-G(5')ppp(5')N mRNA caps during the rate-limiting initiation step of translation. The protein resembles a cupped hand and consists of a curved, 8-stranded antiparallel beta sheet, backed by three long alpha helices. 7-methyl-...
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Early concepts of the structure of ribonucleic acid (RNA) were based largely upon the isolation of the nucleotides of adenine, guanine, uracil, and cytosine from alkali-hydrolyzed RNA (1). Since these were regarded as being single compounds phosphorylated in the 3’ position, and since the acid-stable ribose-5-phosphate (and its pyrimidine derivatives) could not be found in acid-hydrolyzed RNA, ...
متن کاملOn the Structure of Ribonucleic Acids
The complete hydrolysis of ribonucleic acids (RNA) to their constituent mononucleotides, while necessary for determining the composition and also indicative of the nature of the phosphoryl attachments, fails to give any information as to the sequences or subgroup structures existing in the parent molecule. The need and qualifications for a method which will bring about a reproducible degree of ...
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ژورنال
عنوان ژورنال: Microbiological Reviews
سال: 1980
ISSN: 0146-0749
DOI: 10.1128/mmbr.44.2.175-205.1980